ISOLATION AND ANTIBACTERIAL ACTIVITY OF SOIL-DERIVED FUNGI FROM TAMAN BOTANI NEGARA, SHAH ALAM, MALAYSIA
ABSTRACT: Fungi are eukaryotic
organisms that consist of unicellular organisms, namely molds and yeasts, and
multicellular organism known as mushrooms. In the medical field, fungi have a
significant contribution as they are widely used as sources for discovering a
lot of novel antibiotics. As the preliminary study, this paper presents the
isolation of soil-derived fungi from Malaysian forest as resources for finding
the new antibiotics and their test of antibacterial activity against Bacillus
subtilis and Escherichia coli. The fungi their selves were isolated by using Sabouraud
dextrose agar (SDA) medium. The pure fungi isolates were screened for their
antibacterial activity by using disk diffusion method. The active fungi were
fermented in Sabouraoud dextrose broth (SDB) medium for 21 days. Culture media
and mycelium were separated by filtration method. The culture broth was
extracted by liquid-liquid extraction and mycelium was extracted by maceration
method using ethyl acetate. The antibacterial activity of the dried extracts
was determined by using the microdilution method. The isolation step resulted
in five fungal strains coded S1-S5. The antibacterial assay showed that the
extract of fungal broth medium of S3 had the highest antibacterial activity
against B. subtilis with MIC value of 64 μg/mL and S1 against E. coli with MIC
value of 32 μg/mL. Based on these MIC values, these can be classified as
significant antibacterial activities as well. Thus, these extracts could be
potentially useful for the development a new therapeutic agent bacterial
infections.
Keywords: antibacterial
activity, Bacillus subtilis, Escherichia coli, fungi, isolation
Penulis: Marlia Singgih, Elin
Julianti, Muhammad Daniaal Radzali
Kode Jurnal: jpfarmasidd170530
