Isolasi Mikroba Endofit Dari Kulit Batang, Daun, dan Kulit Buah Manggis (Garcinia mangostana L.) Pengkulturan Serta Uji Aktivitas Antimikrobanya
Abstrak: The antimicrobial
activity of endophytes microbe that symbiosis with mangosteen had been carried
out. There were 12 fungal’s isolates and
14 bacterial’s isolates had been isolated from cortex, lief, and pericarp of Garcinia
mangostana L. Each fungal’s isolates were fermented in Soybean Extract Broth
liquid medium for 1 week at on a rotary shaker incubator at a 25-270 C and 90
rpm speed. Each bacterial’s isolates were fermented in water corn soaked medium
for 24 hours on a rotary shaker incubator at 300C and 120 rpm speed. Fermented
fungal’s and bacterial’s were tested their antimicrobial’s activity against
Staphylococcus aureus, Escherichia coli and Candida albicans with agar
diffusion methode. The result showed that J-G1 (Candida sp) and J-T1 (Candida
sp) were fungal’s isolates that ware active against Escherichia coli with
inhibition diameter 10 mm. J-H1 (Curvularis sp), J-G2 (Candida sp), J-T1
(Candida sp) were fungal’s isolates that were active against Staphylococcus
aureus with inhibition diameter 9 mm, 11 mm and 13 mm respectively. B-T2
(Klebsiella sp)and B-K51 (Bacillus sp1) were bacterial’s isolates that were
active againts Escherichia coli with inhibition diameter 13 mm and 18 mm
respectively. B-H (Bacillus sp2), B-G (Bacillus sp2), B-P1 (Bacillus sp1),
B-K101(Bacillus sp1) and B-K102 (Bacillus sp1) were bacterial’s isolates that
were active againts Staphylococcus aureus with inhibition diameter 11 mm, 14
mm, 15 mm, 16 mm and 15 mm respectively.
Kata Kunci: isolation;
Endophytes microbe; mangosteen; antimicrobial
Penulis: Akmal Djamaan, Asia
Asia, Rina Wahyuni
Kode Jurnal: jpfarmasidd140515