ISOLASI DAN IDENTIFIKASI SENYAWA ANTIBAKTERI EKSTRAK N- HEKSAN DAUN JATI (Tectona grandis L.F)
Abstrak: Research about
Isolation and identification of jati leave extract have been done. The purpose
of this research is to isolate and identify the antibacterial compound of jati leave.
The sample was used in this research obtained from Pharmacognocy-Phytochemistry
Laboratory Islamic State University of
Alauddin Makassar collection.
These sample have
antibacterial activity to Bacillus subtilis, Pseudomonas aeruginosa,
Salmonella typhosa and Staphylococcus epidermidis when conducted by
Bioautography Thin Layer Chromatography (TLC). Second fraction was isolated by
Preparative Thin Layer Chromatography (PTLC) and result 2 bands. The second
isolat tested by Bioautography Thin Layer Chromatography (TLC) and showing
antibacterial activity to Bacillus subtilis, Pseudomonas aeruginosa and
Salmonella typhosa and it was single isolat when treat by multi-eluen TLC and
two dimension. The next step identify the second isolat by spot reagen and
showing positif reaction steroid compound. Ultraviolet spectroscopy
interpretation have maximum wavelength 242 nm and infrared spectroscopy showing
-OH, -CH alifatic, -CO, -CH3, alkanes, alkenes and -C=C.
KATA KUNCI: Isolation;
Identification; Antibacteria; Steroid
Penulis: Andi Armisman Edy
Paturusi, N Nurafianty, Rusli Rusli, Abdul Rahim
Kode Jurnal: jpfarmasidd140547