IDENTIFIKASI DNA LEPTOSPIRA SP PADA SAMPEL AIR DAN TANAH DI KOTA MAKASSAR
ABSTRACT: Leptospirosis is a
zoonotic disease that can be found all over the world, caused by a pathogenic
leptospira genus. This study aims to identify the presence of Leptospira
bacterial DNA in water and soil at several locations in Makassar. This research
was conducted in microbiology laboratory Faculty of Medicine Hasanuddin
University. A total of 16 samples were taken consisting of water and soil
samples taken from four locations in Makassar City namely eggplant market,
slums, TPA (final landfill) tamangappa and slaughterhouse (RPH). Samples were
then tested using the nested RT-PCR method . The results of this study showed
that from 16 samples tested showed one sample with positive to the presence of
Leptospira sp bacteria obtained from the soil found in slums located in
makassar.
Kata Kunci: Leptospirosis,
leptospira, water, soil, nested RT-PCR
Penulis: Muhammad Fadhlullah
Mursalim, Mochammad Hatta
Kode Jurnal: jpfarmasidd180145
