Hepatoprotective Activity of Ethyl Acetate Fraction of Senggugu’s Root Bark (Clerodendrum serratum L.Moon) on Rats Induced by CCl4
Abstract: Senggugu is a plant
that has long been used to treat syphilis, typhoid, cancer, jaundice, and
hypertension. The pharmacological activity of senggugu in Indonesia that have
been reported include antifertility activity in leaves, mucolytic activity,
anti-inflammatory and tracheospasmolytic, also antioxidant in its root bark.
This study aims to determine the hepatoprotective effect of ethyl acetate
extract fraction of senggugu’s root bark in rats induced by CCl4. The powder of
senggugu’s root bark was extracted by terraced maceration method starts from
n-hexane, ethyl acetate, to methanol, thus obtained ethyl acetate extract
fraction of senggugu’s root bark (FEAKAS). The ethyl acetate extract fractions
were then tested for hepatoproctective activity using doses of 25, 50, and 100
mg/Kg.BW on rats induced by CCl4. FEAKAS hepatoprotective activity was
determined from the analysis of blood biochemical and oxidative stress
parameters. The blood biochemical parameters included SGOT (serum glutamic
oxaloacetic transaminase), SGPT (serum glutamic pyruvic transaminase), ALP
(alkaline phosphatase), bilirubin, and total protein were measured with test
kit. The oxidative stress parameters were measured from homogenates of liver
tissue that were prepared by adding 500 mL of 50 nM Tris buffer (pH 7.4)
containing 1 mM EDTA and 10 µg/mL leupeptin. The homogenates were centrifuged
to obtain supernatants for measurement of oxidative stress parameters using
spectrophotometer method, including MDA (malondialdehyde), GPx (glutathione
peroxidase) and CAT (catalase). The results showed that the effect of FEAKAS
against CCl4 induction for preventing lipid peroxidation, from both blood
chemical and oxidative stress parameters, are shown at a dose of 100 mg/Kg.BW
that significantly different compared to CCl4 control (ρ <0.05) on all blood
chemical and oxidative stress parameters.
Keywords: Clerodendrum
serratum; antioxidant; hepatoprotective effect; carbon tetrachloride
Penulis: Nasrudin, Wahyono, Mustofa,
Ratna Asmah
Journal Code: jpfarmasigg170002
