Effect of Guava Extract Administration on Megakaryocytes Amount in Mice Femur
Abstract: Dengue fever is a
disease spread by mosquito’s bite. Dengue fever is marked by the presence of
thrombocytopenia. Traditional crops such as guava are commonly used to treat
dengue fever. This research aims to know the effect of guava extract
administration towards megakaryocytes amount in mice femur. The study was
conducted at the Laboratory of Pharmacology and Therapy, Histology Laboratory
of Faculty of Medicine at Universitas Padjadjaran, Eijkman, Bandung from
September until November 2016 using laboratory experimental study design. 20
Swiss webster mice strains were divided randomly into 4 groups. Group I and II
were administered quinine 2.8 mg/20 grBW/day for 14 days to decrease amount of
trombocytes. Group II and III were administered guava extract 0.785 mg/20 grBW/day
for 5 days. Group IV was administered aquadest for 19 days. In the 27th day,
the mice left femurs were collected and made into paraffin section preparations
with hematoxylin-eosin staining and then observed under microscope. Group IV
had the most megakaryocytes followed by Group II, III, and I. Based on
Kruskal-Wallis test, a significant difference was shown (p<0.05). Mann-
Whitney test showed that there were significant differences between Group I and
Group II, III, and IV. Meanwhile there was no significant difference between
normal mice and extract-given mice. Guava extract is proven statistically
significant to increase the megakaryocytes amount in thrombocytopenic mice
without increasing number of megakaryocytes in normal mice.
Keywords: Dengue fever; Guava
extract; Megakaryocyte; Quinine
Penulis: Nur Atik, Maqrizi D.
S. Munawir, Susan Tarawifa, Hartati P. Darmadji
Kode Jurnal: jpfarmasidd170022
