Cytotoxic Activity and Phytochemical Analysis of Breynia cernua from Papua
Abstrak: Breynia cernua (local
name Katuk Hutan) is an Indonesian medicinal plant (family: Euphorbiace- ae)
originated from Papua which have been used traditionally as alternative
treatment for breast and cervical cancer. The objectives of this research were
to investigate potential cytotoxic activity and phytochemical aspect test of B.
cernua extract and its fractions. Extraction was performed by macera- tion
using ethanol 96%, followed by successive fractionations. Phytochemical
screening and in vitro cytotoxic activity test were obtained for extract and
active fraction with Brine Shrimp Lethality Test (BSLT) method and MCF-7 tumor
cell lines were assessed by 3-(4-5-dimethylthiazol-2yl)-2,5-diphe- nyl
tetrazolium bromide (MTT) assay. The results from cytotoxic activity test of
breast cancer MCF-7 cell line from ethanolic 96% extract and three fractions:
n-hexane, ethylacetate and water fractions showed 246.841 ppm, 165.65 ppm,
562.57 ppm dan 713.78 ppm respectively meanwhile the IC50 value of doxorubicin
as the positive control was 6 μM. TLC and phytochemical screening showed the
pres- ence of alkaloids, terpenoid, avonoids and tannins compounds in this
active plant.
Keywords: Antitumor, Breynia
cernua, BSLT, MCF-7
Penulis: Septriyanto
Dirgantara, Rosye H.R. Tanjung, Hendra K. Maury, Edy Meiyanto
Kode Jurnal: jpfarmasidd180231
