Antitumor Activity of Soursop (Annona muricata L.) Leaves On Prostate Cancer Cell Line
Abstract: Soursop (Annona
muricata L.) leaves were used as a traditional drug for many diseases such as
cancer. Statistical data from one cancer
hospital in Jakarta in the period of 2005 and 2007 showed that prostate cancer
has become one of ten common cancer cases in ambulatory patients. This research
was conducted to investigate the potency of an herbal drug for prostate cancer.
The soursop leaves were extracted by maceration and fractionated by
liquid-liquid extraction. The activity of all samples was then tested by Brine
Shrimp Lethality Test (BSLT) and MTT assay using prostate cell line, LNCaP. The
result showed that soursop leaves extract and all fractions had a cytotoxic
activity to Artemia salina L. larvae (LC50<1000 ìg/ml). The MTT assay showed
that soursop leaves extract, n-hexane and ethyl acetate fractions had a
proliferative effect to LNCaP cell line (IC50<350 ìg/ml). Whereas, water
fraction had no effect up to 400 ìg/ml. Ethyl acetate fraction showed the best
antitumor activity because its LC50 and IC50 values were the lowest. It can be
suggested that soursop leaves showed antitumor activity and had a prospective
to be developed as herbal drug for prostate cancer therapy.
Keywords: Soursop leaves,
Annona muricata L., Brine Shrimp Lethality Test (BSLT), LNCaP
Penulis Ade Zuhrotun, Rizky
Abdullah, Meiliana Thamrin, Resti Febriliza
Kode Jurnal: jpfarmasidd130424
