Antitumor Activity of Soursop (Annona muricata L.) Leaves On Prostate Cancer Cell Line

Abstract: Soursop (Annona muricata L.) leaves were used as a traditional drug for many diseases such as cancer.  Statistical data from one cancer hospital in Jakarta in the period of 2005 and 2007 showed that prostate cancer has become one of ten common cancer cases in ambulatory patients. This research was conducted to investigate the potency of an herbal drug for prostate cancer. The soursop leaves were extracted by maceration and fractionated by liquid-liquid extraction. The activity of all samples was then tested by Brine Shrimp Lethality Test (BSLT) and MTT assay using prostate cell line, LNCaP. The result showed that soursop leaves extract and all fractions had a cytotoxic activity to Artemia salina L. larvae (LC50<1000 ìg/ml). The MTT assay showed that soursop leaves extract, n-hexane and ethyl acetate fractions had a proliferative effect to LNCaP cell line (IC50<350 ìg/ml). Whereas, water fraction had no effect up to 400 ìg/ml. Ethyl acetate fraction showed the best antitumor activity because its LC50 and IC50 values were the lowest. It can be suggested that soursop leaves showed antitumor activity and had a prospective to be developed as herbal drug for prostate cancer therapy.
Keywords: Soursop leaves, Annona muricata L., Brine Shrimp Lethality Test (BSLT), LNCaP
Penulis Ade Zuhrotun, Rizky Abdullah, Meiliana Thamrin, Resti Febriliza
Kode Jurnal: jpfarmasidd130424

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