ANTI-INFLAMMATORY AND ANTI-OXIDANT ACTIVITIES OF GLOCHIDION DALTONII BRANCH EXTRACT
ABSTRACT: Glochidion daltonii
(MÜll. Arg.) Kurz, Euphorbiaceae family, is a native medicinal plant in
tropical regions of Asia. In Thailand, it has traditionally been used for
treating pain and mouth inflammation. Objective: This study aims to investigate
the anti-inflammatory and anti-oxidant effects of G. daltonii ethanolic extract
(GDE) both in vitro and in vivo. Method: The anti-inflammatory mechanism was
examined in E.coli LPS-stimulated RAW264.7 cells by using
semi-quantitative-reverse transcription-polymerase chain reaction and the
potential anti-inflammatory effect of GDE was evaluated in Sprague-Dawley rat
using carrageenan-induced rat paw edema method. In addition, DPPH assay was
used for anti-oxidant evaluation. Result: The results showed that GDE at 0.063
- 0.250 mg/mL concentrations inhibited the expressions of tumor necrosis
factor-α and interleukin-1β. Moreover, an intraperitoneal administration of GDE
significantly reduced paw edema in rats. Furthermore, GDE showed high
anti-oxidant property with IC50 values at 6.35 ± 0.28 µg/mL. Conclusion: The
results support the traditionally use of GDE for treating inflammation.
KEYWORDS: glochidion daltonii;
anti-inflammation; anti-oxidation
Penulis: Jintana Junlatat,
Bungorn Sripanidkulchai
Kode Jurnal: jpfarmasidd160765
