Uji Sitotoksisitas Ekstrak Pektin Limbah Kulit Buah Kopi Robusta (Coffea canephora) pada Kultur Sel Fibroblas Pulpa Gigi Manusia
ABSTRACT: Coffee has a
by-product (waste product) that generated from coffee processing. Coffee
fruit's peels contains active compound, that is pectin. Pectin has several
benefits in health, as a regeneration of hard and soft tissue that can be
applied in dentistry and pectin has the potential as an anti-cancer substance.
Pectin as a biomaterial must have biocompability. Biocompatibility of the
material can be tested by cytotoxicity test by MTT method on human dental pulp
fibroblasts cell culture. Objective: To know the toxicity of extract pectin
from coffee robusta fruit peels on human dental pulp fibroblasts cell culture.
Method: This research is a experimental laboratory with post-test only control
group design. Culture of fibroblast cells at the well was exposed by pectin
extract for 24 hours, then each well was given MTT and incubated for 4 hours.
The effects of cytotoxicity were measured by calculating cell viability (%).
Data were analyzed using Probit test, One-way ANOVA followed by LSD. Result: Each
group has a cell viability value above 50%. Conclusion: Extract pectin from
Robusta coffee fruit peel's had no cytotoxic effect on human dental pulp
fibroblsts cell culture
Keywords: coffe robusta fruit
peels, cytotoxicity, human pulp fibroblst cell culture, pectin
Penulis: Kharishah Muslihah,
Agus Sumono, Dwi Warna Aju Fatmawati
Kode Jurnal: jpkesmasdd180034
