Uji Sitotoksisitas Ekstrak Pektin Limbah Kulit Buah Kopi Robusta (Coffea canephora) pada Kultur Sel Fibroblas Pulpa Gigi Manusia

ABSTRACT: Coffee has a by-product (waste product) that generated from coffee processing. Coffee fruit's peels contains active compound, that is pectin. Pectin has several benefits in health, as a regeneration of hard and soft tissue that can be applied in dentistry and pectin has the potential as an anti-cancer substance. Pectin as a biomaterial must have biocompability. Biocompatibility of the material can be tested by cytotoxicity test by MTT method on human dental pulp fibroblasts cell culture. Objective: To know the toxicity of extract pectin from coffee robusta fruit peels on human dental pulp fibroblasts cell culture. Method: This research is a experimental laboratory with post-test only control group design. Culture of fibroblast cells at the well was exposed by pectin extract for 24 hours, then each well was given MTT and incubated for 4 hours. The effects of cytotoxicity were measured by calculating cell viability (%). Data were analyzed using Probit test, One-way ANOVA followed by LSD. Result: Each group has a cell viability value above 50%. Conclusion: Extract pectin from Robusta coffee fruit peel's had no cytotoxic effect on human dental pulp fibroblsts cell culture
Keywords: coffe robusta fruit peels, cytotoxicity, human pulp fibroblst cell culture, pectin
Penulis: Kharishah Muslihah, Agus Sumono, Dwi Warna Aju Fatmawati
Kode Jurnal: jpkesmasdd180034

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