PROTECTIVE EFFECTS OF CATECHINS ISOLATE FROM GMB4 CLONE GREEN TEA AGAINST EPC IN TYPE 2 DIABETES MELLITUS
ABSTRACT: This study aimed to
evaluate the role of catechins isolates from GMB4 clone green to in the
dynamics of endothelial progenitor cells (EPC) in type 2 diabetes mellitus
(DM). Methods: 25 Wistar rats, were divided into 5 groups, including control
group, type 2 diabetes mellitus group, treated daily administration of extracts
of catechins isolate from GMB4 clones green tea at 20; 40; and 60 mg /kgBB
doses for 6 weeks. EPCs analysis was performed by flow cytometry,levels of NO
was performed using a spectrophotometer, serum levels of SDF-1a was performed
with ELISA technique. Analysis of the expression of SDF-1 and CXCR-4 was
performed using immunohistochemistry techniques. Results : The CD34+ and CD133+
count is lower in the DM group compared to the control group (P < 0.05). Of
the three doses of catechins, only the highest dose were able to significantly
increase CD34+ count compared to rats in the diabetes mellitus group (P <
0.05), Serum NO level is significantly higher in the DM the control group (P
< 0.05). All three doses of catechins were able to significantly increase
the expression of SDF-1a and CXCR4 in the aorta compared to the DM group or the
control group (P < 0.05). Discussion :
It can be concluded that high dose cathecin isolate from GMB-4 clone
green tea (60 mg/kgBB) may trigger the proliferation and maturation of EPCs in
rats with type 2 DM in environment with high level of NO, involving the
interacton between SDF-1a and CXCR4 in the aorta.
KEYWORDS: aorta; catechins;
endothelial progenitor cells; hyperglycemia
Author: Yuly Peristiowati
Journal Code: jpkesmasgg170047
