Optimasi Konsentrasi Span 80 dan Lama Pengadukan dalam Preparasi Microspheres Metformin Hidroklorida-kitosan
ABSTRACT: Metformin
hydrochloride (MH) has been used as first line for type 2 diabetes mellitus
treatment. However, it has relatively low bioavailability, short half-life and
could cause gastrointestinal side effects, therefore it is appropriately
prepared as microspheres. Microspheres is micro particle which is between
1-1,000 µm in size. Many factors affected the result of microspheres
preparation, such as surfactant concentration and stirring time. This research
was aimed to find the best composition of span 80 concentration and stirring
time to produce MH-chitosan (CH) microspheres with the highest entrapment
efficiency (EE) using factorial design optimization. MH was used as active
substance, CH as polymer, span 80 as surfactant and non-aqueous solvent
evaporation method was chosen for microspheres preparation technique. The
optimized formula 2 % span 80 and 2 hours stirring time gained 86.803 ± 0.544 %
EE, 24.571 ± 0.157 % drug loading, and 88.220 ± 0.137 % yield. The microspheres
had a relatively smooth, bright surface, and spherical structure. The average
particle’s size was 161.2 ± 1.743 μm. FTIR analysis indicated that there were
no changes in the spesific functional clusters of MH as an active substance.
Keywords:
metfomin-hydrocloride-chitosan microspheres, non-aquoeous solvent evaporation,
factorial design, span 80 concentration, stirring time
Penulis: Christyn Novita S,
Lusia Oktora Ruma Kumala Sari, Eka Deddy Irawan
Kode Jurnal: jpkesmasdd180014
