Inhibitory Activity Test of Quercitrin Isolate from Masisin Parasite Dendrophthoe pentandra (L.) Miq. Against L1210 Leukemia Cells
Abstract: Parasitic plant
[Dendrophthoe pentandra (L.) Miq.] on the masisin [Rhodomyrtus tomentosa
(Aiton) Hassk.] was traditionally used for cancer treatment in Central
Kalimantan. Maceration of dried Dendrophthoe pentandra using ethanol followed
by ethyl acetate produced ethanol and ethyl acetate extracts which exhibit
inhibitory activity against leukemia L1210 cells with IC50 of 17.3 and 20.9
µg/mL, respectively. Fractionation of ethyl acetate extract by column
chromatography yielded 7 fractions (F1~F7). Inhibitory activity test on
fractions F1~F7 against leukemia L1210 cells showed inhibitory activity with
IC50 0f 19.9, 19.0, 20.7, 16.0, 13.7, 12.5, and 17.4 µg/mL, respectively.
During evaporation of F5 by rotary evaporator, a yellowish precipitate was
observed and identified as quercitrin (quercetin 3-O-rhamnoside). Inhibitory
activity test on quercitrin against leukemia L1210 cell showed that the crystal
has an IC50 of 14.7 µg/mL. Separation of F7 by semipreparative HPLC reverse
phase column yielded isolate A and B which exhibited inhibitory activity on
leukemia L1210 cells with IC50 of 15.0 and 14.4 µg/mL, respectively. Subsequent
solvent partition of ethanol extract into ethyl acetate-water and separation of
ethyl acetatesoluble portion by column chromatography produced 7 fractions
(F8~F14). Further separation of F14 by column chromatography yielded quercitrin
and isolate C which has not been identified yet.
Penulis: ERMIN KATRIN W
Kode Jurnal: jpfarmasidd110196
