Hidrolisis Protein Isolat Biji Melinjo (Gnetum gnemon L.) menggunakan Alkalase Terimobilisasi dan Aktivitasnya sebagai Antihipertensi
ABSTRACT: Hypertension is
ranked third on the cause of death in Indonesia for all ages (6.8 %). Protein
isolate of melinjo seeds (Gnetum gnemon L.) hydrolyzed with free alcalase has
been known to have antihypertensive activity. However, the use of free alcalase
is uneconomical since it can only be used once, thus we studied the immobilized
alcalase effectivity to hydrolise the melinjo seeds protein isolate to be used
as angiotensin converting enzyme (ACE) inhibitor. The immobilization method
used entrapment method with DMDMOS/TMOS as matrix. Characterization of the
immobilized enzyme was observed by FTIR and effectiveness of repeated
hydrolysis was observed by the value of the degree of hydrolysis. The success
of the hydrolysis process was determined by SDS-PAGE electrophoresis.
Antihypertensive activity test was determined by the ability to inhibit ACE.
The results showed that the alcalase has been immobilized in the matrix of
silane and SDS-PAGE protein profile showed melinjo seed proteins had been
successfully hydrolyzed. Hydrolysis process repeatedly demonstrated that
immobilized alcalase was effective to hydrolyze protein isolates of melinjo
seeds twice. Based on ACE inhibitory test, there were no significant differences
between the protein isolate hydrolyzed by immobilized alcalase (Gg-PH), protein
isolates hydrolyzed with free alcalase (Gg-PHB), and captopril. These findings
suggest that the antihypertensive activity in Gg-PH is the same with that of
captopril.
Keywords: hypertension,
antihypertensive, melinjo, enzymes immobilization, ACE inhibitor
Penulis: Nur Fauziah Matra
Kode Jurnal: jpkesmasdd180012
