Determination of Sugar Content in Fruit Juices Using High Performance Liquid Chromatography

Abstract: Sugar is a sweet, water-soluble, crystallizable material which is obtained commercially from sugarcane or sugar beet. As an important source of dietary carbohydrate, a sweetener, preservative in foods and a cause factor for Diabetic disease, analysis of sugar content is needed. The objective of this study was to verify the suitability and determination of sugar content in fruit juices using HPLC. The fresh fruits except strawberries were peeled, cut and blended using homogenizer. After filtration, 12.5mL of each fresh juices and packed juices were diluted with acetonitrile and distilled water (50:50). The diluted fresh juices and packed juices were loaded onto C18 Sep-Pak cartridge. Fructose, glucose, and sucrose of each fresh juices and packed juices were analyzed in HPLC with Refractive Index detector, NH2 polar bonded phase column, 10μm (250mm ×4.6mm I.D.), temperature of 43.5°C, mobile phase of acetonitrile and 10mM sodium phosphate (monobasic) (78:22) and flow rate of 1.0mL/min. The method validation showed good linearity of equations for fructose, glucose and sucrose were y = 3833208.4806x – 94721.0361, y = 3782886.4708x – 101683.4708, y = 3770593.9638x – 82870.9083 with regression coefficients, r2 of 0.9995, 0.9997 and 0.9996 respectively. The calculated regression function coefficients (Vx0) for fructose, glucose and sucrose were 0.0152, 0.0112 and 0.0132% respectively. The percentage recovery of fructose, glucose, and sucrose was found to be in a range of each 86.681 – 89.888, 86.898 – 90.029, and 94.541 – 97.885%. Sugar contents in fruit juices can be determined using verified HPLC method with Refractive Index Detektor. Fructose, glucose and sucrose of fresh juices contained 0.469 – 1.431, 0.454 – 1.286, 0.544 – 1.861%, whereas that of packed juices contained 0.309 – 1.587, 0.261 – 0.762, 0.063 – 0.898%, respectively.
Keywords: Fructose, glucose, sucrose, fruit juice, HPLC
Penulis: Sophi Damayanti, Benny Permana, Choong Chie Weng
Kode Jurnal: jpfarmasidd120336

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