Pengaruh pemberian ekstrak jamur merang (volvariella volvacea) terhadap kadar kolesterol total, enzim lppla2 dan mda darah
Abstract: Consumption of
high-fat diet (HFD) is increasing blood total cholesterol, LpPLA2 enzyme and
MDA level which is a cause of atherosclerosis that should be controlled by
antioxidants. Paddy straw mushroom (Volvariella v.) extract has an antioxidant
compounds. This research proves the effect of Volvariella v. extract on blood
total cholesterol (TC), LpPLA2 enzyme and MDA level, and proves correlation
between TC with LpPLA2 enzyme and MDA level.
Methods: A randomized post test only control group design to twenty four
of 8-10 weeks old male Rattus novergicus
Wistar strain with 180-200 gram were divided into four group; K1 with standard
diet, K2 with HFD, each X1 and X2 with HFD+Volvariella v. extract 500
mg/kg.bwt/day and 1000 mg/kg.bwt/day. All treatments for 56 days. TC levels
assesed by enzymatic colorimetric test method, LpPLA2 enzyme with ELISA method
and MDA with TBARs method. Data were analyzed by one way Anova, LSD post hoc
and Pearson’s correlation.
Results: TC mean of K1 (91,28±1,55 mg/dL), K2 (181,96±4,19 mg/dL), X1
(122,94±2,75 mg/dL) and X2 (105,86±2,25 mg/dL); p=0,001. Enzyme LpPLA2 mean of
K1 (2,65±3,19 ng/mL), K2 (1,29±1,35 ng/mL), X1 (3,92±2,18 ng/mL), X2 (2,05±3,29 ng/mL); p=0,385 and blood MDA
mean of K1 (1,17±0,12 nmol/mL), K2 (5,75±0,21 nmol/mL), X1 (2,96±0,91 nmol/mL)
and X2 (1,77±0,92 nmol/mL); p=0,001. Correlation between TC and LpPLA2 enzyme
was shown by X1 group (p=0,042; r = - 0,827).
Conclusion:Volvariella v. extract decreased blood total cholesterol and
MDA level significantly, did not significant to LpPLA2 enzyme. There wss a
correlation between TC and LpPLA2 enzyme but was not to TC and blood MDA.
Keywords: Diet tinggi lemak;
kolesterol total; enzim LpPLA2; MDA; ekstrak jamur merang
Penulis: Amelia Eka Damayanty,
Lisyani B. Suromo, RA Kisdjamiatun
Kode Jurnal: jpkesmasdd150749
