Y-Chromosomal Microdeletion in Idiopathic Azoospermic and Severe Oligozoospermic Indonesian Men
Abstract: to detect
Y-chromosomal microdeletion in Indonesian men with azoospermia or severe
oligozoospermia using multiplex PCR. Methods: we performed 2 multiplex PCR
amplifications of the Azoospermia Factor (AZF) region in 71 men. Criteria for
including a patient were fulfilled if they presented with azoospermia or severe
oligozoospermia, with or without additional abnormalities of sperm motility or
of head morphology, raised or normal levels of FSH, normal levels of LH and
testosterone, and with no evidence of testicular tumors or other abnormalities.
Five men participated as control persons. Results: partial deletion of AZFa was
found in 11 men (15.49%), complete deletion of AZFb in 1 man (1.4%), and
complete deletion of AZFc in 1 man (1.4%). The unspecific type of deletion was
also detected, including the DBY gene in 2 men (2.81%), and partial deletion of
both AZFa and AZFb in 2 men (2.81%). No AZF deletion was observed in the
control probands. Related to the type of deletion, the AZFa and AZFb deletion
showed spermatogenesis arrest in most tubules, while deletion of the DBY gene
is associated with the sertoli cell only (SCO) syndrome. Conclusion: the
frequency of partial deletion of AZFa was found to be relatively high in our
center. The type of deletion is associated with the testicular histology.
Keywords: azoospermia; male
infertility; microdeletion; multiplex polymerase chain reaction; Y-chromosome
Author: Ponco Birowo, Donny
Eka Putra, Mewahyu Dewi, Nur Rasyid, Akmal Taher
Journal Code: jpkedokterangg170323
