Profile of PRMT-1 Gene Polymorphism in Hemodialysis Patients with Increased ADMA Levels
Abstract: to determine the
distribution of PRMT-1 gene polymorphism and ADMA levels among continuing
hemodialysis patients. Methods: genotyping of PRMT-1 polymorphism was performed
in 57 hemodialysis patients at Al Irsjad Hospital. All participants were
recruited for physical examination, questionnaire, and collection of 5 mL
fasting venous blood. The blood was treated with phenol-chloroform extraction
of genomic DNA. The candidate’s single nucleotide polymorphisms (SNPs) were
genotyped by using polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP). The ADMA plasma levels was determined by ELISA and all
biochemical indicators of serum were examined. Results: fifty-seven
hemodialysis patients participated in our study, 54 (95.4%) of them had
increased ADMA plasma levels. DNA sequencing analysis of 13 patients showed a
suspected PRMT-1 gene polymorphism at sequence 5837 as there were different
genotypes between C and G. Conclusion: the increased levels of ADMA might be
caused by PRMT-1 gene polymorphism.
Key words: ADMA, PRMT-1
polymorphism, chronic kidney disease
Author: Mochammad Thaha, Wenny
P Nilamsari, Mochammad Yusuf, M. Amin
Journal Code: jpkedokterangg140308
