Level of Circulating Endothelial Cells and Expression of Nuclear Factor Kappa Beta of Human’s Peripheral Blood Mononuclear Cells in Subjects with Certain Conditions
Abstract: to detect the levels
of CEC and expression of NFκB in the three groups of subjects with certain
conditions. Methods: this study is an exploratory study using human peripheral
blood samples. The study subjects comprised three groups, the group of 23
healthy people, a group of 35 people that has one or more risk factors for
vascular disease and a group of 15 vascular disease patients (coronary heart
disease, diabetes mellitus, stroke). CEC were isolated from peripheral blood
mononuclear cells (PBMC). CEC level is identified through the measurement of
CD45 and CD146 by flowcytometry method. NFκB expression is recognized by ELISA
method (imgenex, USA). Results: the highest average levels of CEC were found in
the sick group (28.6%). The highest average expression of NFκB (924.9495) is
found in the group with risk factors. The lowest average expression of NFκB and
CEC is found in the healthy group. Statistical analysis of ANOVA at the
interval confidence of 95% shows a significant difference (p=0.00) levels of
CEC and NFκB expression between the healthy group with the group with the risk
of cardiovascular disease (CVD) and patients with known CVD. Conclusion:
increase of level CEC and NFκB expression has a strong relationship with
vascular disease and its risk factors.
Key words: circulating
endothelial cells (CEC), nuclear factor kappa beta (NFκB), peripheral blood
mononuclear cell (PBMC), vascular disease
Author: Titin A Wihastuti,
Djanggan Sargowo, Achdiat Agoes, Satuman, Teuku Heriansyah
Journal Code: jpkedokterangg140297
