Gallstone Analysis
Abstract: Gallstone is a
crystal deposit which is formed in the gallbladder or bile duct. Gallstone is
classified into cholesterol stone, pigment stone (black and brown), and mixed
stone. Mechanism which underlies the formation of cholesterol or pigment
gallstone is different. Information on chemical component of the stone will
assist the management and prevention of its recurrence. Analysis of gallstone
component can be performed by colorimetry method or even gas liquid
chromatography (GLC). Chemical component analysis of gallstone by colorimetry
includes examination of cholesterol, bilirubin, and calcium. Stone is
classified as cholesterol stone if the cholesterol content is > 80%, pigment
stone if cholesterol content is < 20%, and mixed stone if cholesterol
content is 25-80%. Gallstone analysis by GLC method is conducted by separation
of fatty acid chain and evaluation of fatty acid quantity in the methylester
derivatives form, which is fatty acid methyl estered. Fatty acid content in
cholesterol stone (310.09 + 49.7 mg/gram) is higher compared to pigment stone
(55.59 +7.71 mg/gram). Saturated to unsaturated fatty acid (S/U) ration in
cholesterol stone (8.6 + 3.1) is higher compared to pigment stone (4.8 + 1.5).
Keywords: gallstone;
colorimetry; gas liquid chromatography
Author: Fransiska Putri, Nuri
Dyah Indrasari
Journal Code: jpkedokterangg160413
