Detection of Helicobacter pylori CagA gene and Its Association with Endoscopic Appearance in Balinese Dyspepsia Patients
Abstract Helicobacter pylori
(H. pylori) infection causes various abnormalities in the stomach. Only
particular strain can cause severe problems in the stomach. CagA is a microbial
virulent factor which is associated with more severe stomach problems, such as:
peptic ulcer and stomach cancer. We would like to know the prevalence of CagA
in Balinese population, and the association of H. Pylori CagA status with the
severity of endoscopic appearance in dyspepsia patients.
Method: Study design being used was analytic cross sectional study,
involving 71 dyspepsia patients who underwent upper gastrointestinal endoscopic
examination in Surya Husada Hospital and Balimed Hospital in June-December
2013. Sample was chosen in consecutive manner. Later, polymerase chain reaction
(PCR) examinations of the stomach mucous biopsy tissue to determine H. pylori
infection status and CagA status were performed. Further, Chi square test was
used to identify the difference in proportion of H. pylori and CagA between
mild and severe endoscopic appearance.
Results: In this study, we found that the prevalence of H. pylori
infection was 22.5% using PCR examination. Prevalence of CagA positive in H.
pylori positive was 62.5%. There was significant association between status of
H. Pylori infection and severity of endoscopic appearance (p = 0.038; OR= 2.67;
95% CI = 1.18-6.05). Status of CagA in H. pylori infected patients was not
associated with the severity of endoscopic appearance. Additionally, there was
significant association between patients’ age and severity of endoscopic
appearance.
Conclusion: The prevalence of CagA in H. pylori positive was 62.5%. H.
pylori infection was associated with severity of endoscopic appearance and CagA
status in H. pylori infected patients was not associated with severity of
endoscopic appearance.
Keywords: Helicobacter pylori;
CagA; endoscopic appearance
Author: I Ketut Mariadi, I
Dewa Nyoman Wibawa, Ida Bagus Nyoman Wibawa
Journal Code: jpkedokterangg160411
