Variasi Genetik Itik Bayang Berbasis Marka Mikrosatelit Pada Lokus AY287 dan Lokus AY283
Abstrak: West Sumatera
Province has poultry genetic resource of local duck that potential in supply eggs
and duck meat. Bayang duck was set by Indonesian Agricultural Ministry in 2012
as national livestock breeds in Indonesia. Microsatellite markers are widely
used as a genetic identifier because of their abundant existence, co-dominant
and high polymorphic. The purpose of this study was to determine the genetic
diversity of Bayang ducks based on two microsatellite loci which include AY287
and AY283. DNA substances used in the study were blood samples from 24 Bayang
duck in Pesisir Selatan Regency. The isolated DNA genom from 24 blood samples
of Bayang duck could be detected by gel electrophoresis. Results showed that
AY287 locus has 6 alleles; allele A (108 bp), allele B (142 bp), allele C (183
bp), allele D (227 bp), allele E (272 bp) and allele F (340 bp). Both allele E
and F were specific genetic markers of Bayang duck. Alleles frequencies of the
AY287 locus were as follow: allele C (26,93%), allele D (19,24%), allele A
(15,38%), allele B (15,38%), allele E (15,38%) and allele F (7,69%). The AY283
locus has 6 alleles consisted of allele A (230 bp), allele B (320 bp), allele C
(345 bp), allele D (390 bp), allele E (450 bp) and allele F (500 bp). Allele
frequencies of this marker were allele B (20,51%), allele D (20,51%), allele E
(20,51%), allele A (15,39%), allele C (15,39%), and allele F (7,69%),
respectively. Our finding suggest that two microsatellite markers, AY287 and
AY283, were polymorphic in Bayang duck population.
Key words: Bayang duck,
microsatellite, AY283, AY287
Penulis: Rusfidra, Y. Heryandi, Jamsari Jamsari, E. Y.
Rahman
Kode Jurnal: jppeternakandd130397
