The Use of Lactic Acid Bacteria as Ruminant Probiotic Candidates Based on In Vitro Rumen Fermentation Characteristics
Abstract: This research was
conducted to select, to identify LAB isolates and to investigate the effects of
the LAB as probiotics candidate in the rumen fermentation. Nine isolates
exhibited the potency as candidate probiotics for cattle. The experiment was
arranged in randomized block design with ten treatments and three different
times of in vitro as a block. The substrate consisted of 70% forage and 30%
concentrate proportion. The substrate was incubated at 39oC using serum bottle
of 100 ml capacity for fermentation. Approximately 0.75 g of substrates was put
inside the serum bottle glass and filled with 73 ml of buffered rumen fluid and
2 ml of LAB inoculant. Gas production was measured every 2, 4, 6, 8, 10, 12,
24, 48 and 72 h of the incubation period. Gas production kinetic was estimated
by the Ørskov’s equation. The LAB with the highest gas production, as
probiotics candidate, were identified using partial 16S rDNA sequence. The
results of this research indicated that nine LAB produced high gas production
in the range of 193-198 ml compare to that of control (173 ml). The addition of
LAB in rumen fermentation resulted in digestibility 65-75%, organic matter
digestibility 51-73%, and 6.67-6.68 pH. Based on the molecular identification,
8 isolates are Lactobacillus plantarum and 1 of uncultured bacteria. The LAB
strain 32 L. plantarum showed the best for a ruminant probiotic candidate based
on the in vitro rumen fermentation characteristic.
Keywords: Fermentation; Lactic
acid bacteria; Rumen; Probiotics; 16S rDNA sequence
Penulis: Roni Ridwan, Win
Ariga Bungsu, Wulansih Dwi Astuti, Rohmatussolihat, Nurul Fitri Sari, Rusli
Fidriyanto, Anuraga Jayanegara, Indah Wijayanti, Yantyati Widyastuti
Kode Jurnal: jppeternakandd180011
