The suppression of manganese superoxide dismutase decreased the survival of human glioblastoma multiforme T98G cells
Abstract: Glioblastoma
multiforme (GBM) is a primary malignant brain tumor which has poor prognosis.
High incidence of oxidative stress-based therapy resistance could be related to
the high antioxidant status of GBM cells. Our previous study has reported that
manganese superoxide dismutase (MnSOD) antioxidant expression was significantly
higher in high grade glioma than in low grade. The aim of this study was to
analyze the impact of MnSOD suppression toward GBM cell survival.
Methods: This study is an experimental study using human glioblastoma
multiforme T98G cell line. Suppression of MnSOD expression was performed using
in vitro transfection MnSOD-siRNA. The MnSOD expression was analyzed by
measuring the mRNA using real time RT-PCR, protein using ELISA technique, and
specific activity of enzyme using inhibition of xantine oxidase. Concentration
of reactive oxygen species (ROS) intracellular was determined by measuring
superoxide radical and hydrogen peroxide. Cell survival was analyzed by
measuring viability, proliferation, and cell apoptosis.
Results: In vitro transfection of MnSOD-siRNA suppressed the mRNA,
protein, and specific activity of MnSOD. This treatment significantly increased
the concentration of superoxide radical; however, it did not influence the
concentration of hydrogen peroxide. Moreover, viability MnSOD-suppressing cell
significantly decreased, accompanied by increase of cell apoptosis without
affecting cell proliferation.
Conclusion: The suppression of MnSOD expression leads to decrease glioblastoma
multiforme cell survival, which was associated to the increase of cell
apoptotic.
Keywords: Cell survival;
Glioblastoma multiforme; MnSOD-siRNA; ROS; T98G cells
Author: Novi S. Hardiany,
Mohamad Sadikin, Nurjati Siregar, Septelia I. Wanandi
Journal Code: jpkedokterangg170198
