The role of genetic variation in TCF7L2 and KCNJ11, dietary intake, and physical activity on fasting plasma glucagon-like peptide-1 in male adolescents
Abstract: Transcription factor
7-like 2 (TCF7L2) and potassium voltage-gated channel subfamily j member 11
(KCNJ11) gene polymorphisms have been associated with type 2 diabetes mellitus
(T2DM) via regulation of insulin production. Ingested nutrients induce
glucagon-like peptide-1 (GLP-1), which in turn induces insulin secretion.
Objective To evaluate the relationship between TCF7L2 and KCNJ11 gene
polymorphism, dietary intake, and physical activity on fasting plasma GLP-1 in
normal male adolescents.
Methods This observational study with a cross-sectional design included
54 male adolescents selected from high schools in Yogyakarta, Indonesia.
Interviews were done to collect data on energy intake and physical activity.
The GLP-1 and insulin levels were measured from fasting blood plasma. The TCF7L2
and KCNJ11 gene polymorphisms were analyzed by polymerase chain
reaction-restriction fragment length polymorphism (PCR-RFLP).
Results Fasting GLP-1 was positively correlated with energy intake
(r=0.276; P=0.047), but not with physical activity (r=0.011; P=0.936). The
GLP-1 concentration was not associated with TCF7L2 and KCNJ11 gene
polymorphisms (all P>0.05). In subjects with an EE genotype (KCNJ11), GLP-1
was not correlated with insulin (r=-0.036; P=0.435). However, in subjects with
an EK genotype (KCNJ11), GLP-1 was positively correlated with insulin (r=0.394;
P=0.026).
Conclusion GLP-1 concentration is positively correlated with body weight.
Among male adolescents with a genetic variation in KCNJ11 (EK genotype), there
is a significant correlation between GLP-1 and insulin signalling.
Keywords: KCNJ11; TCF7L2;
GLP-1; diet; physical activity
Author: Harry Freitag Luglio,
Emy Huriyati
Journal Code: jpkedokterangg170200
