The pathogenecity of H5N1 highly pathogenic Avian Influenza (HPAI) virus clade 2.3.2. in Indonesian indigenous chicken by contact tranmission with infected duck
Abstract: An experimental
transmission study was conducted using nine healthy Indonesian indigenous
chickens placed together with two 30 days old ducks which were experimentally
infected with H5N1 HPAI clade 2.3.2 virus in the Biosafety Laboratory Level 3
(BSL-3) facilities. The aim of the study was to find out the pathogenicity of
H5N1 HPAI virus clade 2.3.2 in Indonesian indigenous chickens. The study showed
that within twenty four hours rearing, the chickens were exhibited mild
clinical signs and by 48 hours, all of the chickens died, whereas the ducks
survived but with severe clinical signs. The H5N1 HPAI virus has been
successfully isolated from chickens and ducks swabs, confirming that those
animals were infected by the virus. Histologically, the infected chicken
encountered with severe inflammation reaction namely non suppuratives
encephalitis, tracheitis, myocarditis, interstitial pneumonia, hepatitis,
proventriculitis, enteritis, pancreatitis, nephritis and bursitis. Necrotizing
spleen and pancreas were also prominent. Viral antigen was detected by
immunohistochemistry staining in various affected visceral organs. This
suggests that Indonesian indigenous chickens were susceptible to H5N1 HPAI
virus clade 2.3.2 and it can be transmitted easily to Indonesian indigenous
chickens by contact transmission with infected ducks.
Keywords: Pathogenicity; H5N1
HPAI virus clade 2.3.2; Indonesian indigenous chickens
Author: R. Damayanti, A.
Wiyono, H. Nuradji, M. I. Cahyono
Journal Code: jppeternakangg170014