The expression of heat shock protein 70 gene with organic selenium supplementation and its effetc on productivity of broilers in tropical environment
Abstract: The purpose of this
experiment is to study the effect of organic selenium (Se) supplementation on
the expression of heat shock protein 70 gene (HSP70), glutathione peroxidase
(GSH-Px) enzyme activity, malondialdehyde (MDA) and productivity of broilers in
tropical environment. Three kinds of environmental pens were designed in this
experiment: comfortable environment pens with temperature of air conditioner
adjusted at 22oC (R0), tropical environment pens (±30oC ) without organic Se
(R1), and tropical environment pens supplemented with 0.30 ppm organic Se (R2).
One hundred and twenty broiler chickens (unisex) were used in this study. There
were 40 chicks per pen for each treatment. The experimental design was
completely randomized with four replications for each treatment. The data were
statistically analyzed using the general linear model of SAS program. Results
showed that R0 and R2 groups had significantly increased (P<0.05) feed intake,
body weight, body weight gain, and decreased feed conversion ratio compared to
R1 groups. Meanwhile, the expression of HSP70, GSH-Px enzyme activity and MDA
of R2 groups and R0 groups were significantly lower (P<0.05) than that of R1
groups. It was concluded that the broilers given 0.30 ppm organic Se in
tropical environment had similar productivity and expression of HSP 70 with
broilers kept in comfortable environment.
Keywords: broiler; HSP70 gene;
organic selenium; productivity; tropical environment
Author: R. Amizar, S. Suharti,
J. Jakaria, R. Mutia
Journal Code: jppeternakangg170047