The effects of quercetin on oxidative stress and fibrosis markers in chronic kidney disease rat model
Abstract: Oxidative stress may
play a role in the pathogenesis of (CKD), Nuclear factor erythroid 2-related
factor 2 (Nrf2) is a transcription factor involved in cell defense mechanism
against oxidative stress. In this study, we examined the effect of quercetin, a
polyphenplic antioxidant anti fibrosis compund in fruits and vegetables, on the
5/6 nephrectomy-induced CKD progression model rats through modulation of Nrf2
expression.
Methods: Male Sprague-Dawley rats were randomly divided into normal
control group (C), untreated 5/6 nephrectomy (Nx), quercetin-treated 5/6
nephrectomy (100 mg/kgBW/day orally) (NxQ), and captopril-treated 5/6
nephrectomy (10 mg/kgBW/day orally) (NxK) for 8 weeks. At the end of study, all
animals were sacrified. Urine, blood, and kidney tissues were taken for
examination of proteinuria, plasma creatinine, urea, malondialdehyde (MDA),
glutathione peroxidase (GPx) activity, Nrf2, Keap1, heme oxygenase-1 (HO-1)
expressions, and renal fibrosis.
Results: Quercetin administration did not affect the level of protein in
urine, plasma creatinine, and urea. However, it tended to reduce the level of
MDA, increase GPx activity, Nrf2, Keap1, and HO-1 expression as well as the
degree of fibrosis.
Conclusion: In 5/6 nephrectomized rats, quercetin tended to ameliorate
the level of MDA, GPx activity, Nrf2, Keap1, and HO-1 expression. In addition,
quercetin tended to decrease the degree of fibrosis in the remnant kidney.
Keywords: chronic kidney
disease; nephrectomy; Nrf2; oxidative stress; quercetin
Author: Kamalia Layal, Ika S.
Perdhana, Melva Louisa, Ari Estuningtyas, Vivian Soetikno
Journal Code: jpkedokterangg170191
