The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin
Abstract: The objective of the
research was to identify the effect of the concentration of hydrochloric acid
(HCl) on yield and the characteristics of gelatin from buffalo hide. A
completely randomized design was used with various concentrations of HCl
treatment 0.3M, 0.6M, 0.9 M, 1.2 M and 1.5M, and four times replication,
followed by least significant difference test. The HCl concentration gave
significantly effect (P<0.05) on yield, viscosity, pH, gel strength and
redness of gelatin. The highest yield (29.17%) was obtained at a concentration
of HCl 0.9M. The highest gel strength and viscosity at concentration of 1.2M,
were 293.41g bloom and 22.17 cp, respectively. The pH of the gelatin were 5.08
to 5.52. The lightness level (L*) was 69.92-70.97, whereas the redness ranged
(a*) from 0.55 to 1.54 and the
yellowness ranged (b*) from 17.54 to 19.59. The moisture, protein and ash
concentrations of gelatin were 7.05±0.07-8.92±0.06%, protein content of
83.38±0.06 -91.11±0.03%, and the ash content 0.53 ± 0.04 to 1.23 ± 0.09%,
respectively. Based on the spectra absorption of infrared were detected four
areas amides: amide A (3402.43-3448.72 cm-1), amide I (1635.65 cm-1), amide II
(1527.62 cm-1) and amide III (1242.16 cm-1). The differences in the concentration
of HCl had impact on yield,characteristics and secondary structure of buffalo
hide gelatin. The HCl concentration at 0.9M was the optimum condition to obtain
the highest yield. The characteristics of buffalo hide gelatin after
pretreatment using hydrochloric acid had met the standards of GMIA (2012).
Keywords: Hydrochloric acid;
gelatin; buffalo hide; pretreatment; yield
Author: S. Mulyani, F. M. C.
S. Setyabudi, Y. Pranoto, U. Santoso
Journal Code: jppeternakangg170013
