The effect of Bifidobacterium animalis lactis HNO19 supplementation among pregnant and lactating women on interleukin-8 level in breast milk and infant’s gut mucosal integrity
Abstract: Newborn’s gut
mucosal is not fully developed, therefore infants are prone to diarrhea.
Probiotic supplementation is known to induce the gut mucosal maturity. This
study aimed to identify whether probiotics supplementation among pregnant women
since the third trimester would increase the infant’s gut mucosal integrity.
Methods: A double-blind, randomized clinical trial was conducted to
understand the potential effect of probiotic supplementation on the level of
probiotics and IL-8 in breastmilk, urine IFABP, faecal α-1-antytripsin (AAT)
and calprotectin in infant’s at birth (V0) and three-months old (V3). A single
strain of Bifidobacterium lactis animalis HNO19 (known as DR10) was used since
it was not the resident bacteria. The study was held at Budi Kemuliaan Hospital
and its satellite clinics from December 2014 to December 2015.
Results: About 14% (5/35) and 20% (7/35) of the subjects had DR10 in the
breastmilk’s colostrum and at the age of 3-months. The median values of IL-8 in
the probiotic group vs the placebo group at V0 and V3 were 2810,1 pg/mL vs
1516.4 pg/mL (p=0.327) and 173.2 pg/mL vs 132.7 pg/mL (p=0.211) respectively.
IFABP level 211.7 ng/mL vs 842.5 ng/mL (p=0.243) and 25.3 ng/mL vs 25.1 ng/mL
(p=0.466); AAT 136.2 mg/dL vs 148.1 mg/dL (p=0.466) and 24 mg/mL vs 29.72 mg/mL
(p=0.545); Calprotectin 746.8 ng/mL vs 4645.2 ng/mL (p=0.233) and 378.6 ng/mL
vs 391.3 ng/mL (p=0.888).
Conclusion: Probiotic DR10 given to pregnant women since the 3rd
trimester can be found in colostrum and 3-months breastmilk. However, it did
not affect the level of other probiotics or IL-8 and the gut mucosal integrity.
Keywords: Bifidobacterium
lactis animalis HNO19; breastmilk; gut mucosal integrity
Author: Naomi E.F. Dewanto,
Agus Firmansyah, Ali Sungkar, Nani Dharmasetiawani, Sudigdo Sastroasmoro, Siti
B. Kresno, Rulina Suradi, Saptawati Bardosono, Dwi Prasetyo
Journal Code: jpkedokterangg170194
