The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross
Abstract: The chicken growth
hormone (GH) and its receptor (growth hormone receptor, GHR) play important
roles in chicken performances due to their crucial functions in growth. The
variations of GH and GHR genes were then thought to be associated with the
variations of the performances. This experiment was designed to identify the
g.2248G>A GH and the g.565G>A GHR loci polymorphisms and to evaluate
their associations with carcass components in Kampung and broiler chicken
cross. A total of 215 chickens including 4 chicken populations (Kampung, Cobb
broiler, F1, and F2 Kampung x broiler chicken cross) were screened to identify
polymorphism using PCR-RFLP technique with EcoRV and Eco72I restriction enzyme
for GH and GHR loci, respectively. The carcass components were recorded at 26
weeks of age on F2 Kampung and broiler chicken cross (42 chickens) for
association study. Both the g.2248G>A GH and the g.565G>A GHR loci were
polymorphic with two alleles (G and A) and three genotypes (GG, AG, and AA).
The GG genotype and the G allele of GH locus were predominant in all chicken
populations. While in GHR locus, the AA genotype and the A allele were found to
be higher in all chicken populations. The association study showed that the
g.565G>A GHR locus polymorphism had significant effect on carcass
components, including live weight, carcass weight, breast weight, thighs
weight, breast muscle weight, and thighs muscle weight. There was no
significant association was found between the g.2248G>A GH genotype and
carcass components. It could be concluded that the g.2248G>A GH and the
g.565G>A GHR loci were polymorphic in Kampung and broiler chicken cross and
the g.565G>A GHR locus was accosiated with carcass components. This
g.565G>A GHR SNP might be an important candidate marker for chicken growth
and muscle mass improvement.
Keywords: Kampung chicken
cross; growth hormone; growth hormone receptor; gene polymorphisms; carcass
Author: I. Khaerunnisa,
Jakaria Jakaria, I. I. Arief, C. Budiman, C. Sumantri
Journal Code: jppeternakangg170064