Sexual dimorphism and identification of single nucleotide polymorphism of growth hormone gene in muscovy duck
Abstract: This research was
aimed to investigate the different growth and to identify growth hormone gene
polymorphism in Muscovy ducks. Two hundred Muscovy day-old ducks consisting of
white-plumed male and female duck, black and white-plumed male and female
ducks. Body weight was recorded weekly and the obtained data were subject to T
test. Primer design used the Custal X Program based on a database from the
GeneBank Cairina moschata GH gene, partial cds (AB158762). Primer base sequence
of GH gene was forward/Sequence: 5’-CTGGGGTTGTTTAGCTTGGA-3’ and
reverse/Sequence: 5’-TAAACCTTCCCTGGCACAAC-3’. The DNA sequences were aligned by
using the BioEdit version 7.7 for identification of the single nucleotide
polymorphism. The result showed that male Muscovy duck produced higher an
average body weight gain and more relative growth than those of females. The
highest body weight gain was at three weeks old, and then it started to
decrease at four weeks old. The sequencing PCR product obtained nucleotide
polymorphism. AA genotype was observed at 136 t of black female Muscovy duck,
CC in black and white male Muscovy duck, and white female Muscovy duck.
Conclusively, a body weight gain of 3-week-old male Muscovy ducks was higher
than that of females and GH gene polymorphism was observed in Muscovy ducks.
Keywords: growth hormone
polymorphism; Muscovy duck; sexual dimorphism
Author: I. Ismoyowati, E.
Tugiyanti, M. Mufti, D. Purwantini
Journal Code: jppeternakangg170032