SAPODILLA (MANILKARA ZAPOTA) BROTH AS AN ALTERNATIVE MEDIA FOR CANDIDA ALBICANS
ABSTRACT: To determine whether
sapodilla can be used to grow Candida albicans. Among all the high galactose
and arabinose content fruits, the sapodilla was chosen because it is available
year round and can get easily in market. Other than that, it also contains
vitamins, calcium and phosphorus which are very useful for fungi growth.
Methods: This study used an experimental study as a method of research.
The researcher culture Candida albicans on the experimental sapodilla media and
identifies the morphology of the fungi by using Gram staining method. The
experiment will be replicated two times to get accurate result. The procedure
of this experiment constitute of sapodilla media preparation, sapodilla media
observation, organism preparation, planting and incubation, observation of
fungal colonies and identification of the fungi.
Results: In 0%, there was no fungal growth at all. In 5%, there was mild
density of fungal colonies. In 10%, there was moderate density of fungal
colonies and in 15% the fungal grew with very dense colonies.
Conclusions: Sapodilla (Manilkara zapota) broth can be used as an
alternative media for Candida albicans.
Keywords: Alternative media,
Candida albicans, Sapodilla broth
Penulis: Chen Chui Ying, Ine
Kuswardinah, Elsa Pudji Setiawati
Kode Jurnal: jpkedokterandd170225
