Recombinant vascular endothelial growth factor 121 decreases vascular cell adhesion molecule-1 in murine pre-eclampsia model placenta
Abstract: Preeclampsia is one
of the major contributors to maternal and fetal morbidity and mortality.
Imbalance of soluble Fms-like tyrosine kinase (sFlt-1) as anti-angiogenic
factor and vascular endothelial growth factor (VEGF) as pro-angiogenic factor
plays a role in the pathogenesis of preeclampsia. Endothelial dysfunction in
preeclampsia causes vascular cell adhesion molecule-1 (VCAM-1) to be expressed
on its surface. This study aims to evaluate the effect of recombinant VEGF-121
on VCAM-1 expression in the placenta of a murine preeclampsia model.
Methods
An experimental analytical study conducted from February until March 2016
in the Biomedical Laboratory, Faculty of Veterinary Medicine, Airlangga
University. The study sample consisted of 30 pregnant mice, divided into three
groups, i.e. 10 normal pregnant mice, 10 mice with preeclampsia model and 10
mice with preeclampsia model and recombinant VEGF-121 therapy. All animals were
subjected to immunohistochemical examination of VCAM-1 expression in their
placentas. The results were assessed semiquantitatively according to a modified
Remmele method. Data analysis was done using one-way ANOVA and Tukey’s multiple
comparisons method.
Results
Mean VCAM-1 expression in normal (0.97 ± 0.54%) murine placentas, compared
with placentas (2.94 ± 0.96%) of murine preeclampsia models (p=0.000), while
mean VCAM-1 expression in placentas of murine preeclampsia models with VEGF
intervention was 2.14 ± 0.68% (p=0.030).
Conclusion
Recombinant VEGF-121 can reduce VCAM-1 expression in placentas of murine
preeclampsia models. The present study has shown the potential benefits of VEGF
therapy, justifying serious consideration of this therapeutic approach for use
in women with preeclampsia.
Keywords: Recombinant
VEGF-121; VCAM-1; preeclampsia; pregnant murine
Author: Sri Sulistyowati, John
Arianto Sondakh, Eric Edwin Yuliantara, Supriyadi Hari Respati, Soetrisno
Soetrisno
Journal Code: jpkedokterangg160213
