PENGARUH PENGHILANGAN RAFINOSA DALAM PENGENCER TRIS AMINOMETHANE KUNING TELUR TERHADAP KUALITAS SEMEN KAMBING BOER SELAMA SIMPAN DINGIN
Abstract: The objective of
this research was to examine the effect of removal raffinose in tris
aminomethane egg yolk diluents to semen quality of Boer goat during chilled
preservation. This research was carried out at Animal Reproduction Laboratory
and Sumber Sekar Laboratory, Animal Husbandry Faculty, Brawijaya University
from December 11th 2016 to January 31th
2017. Semen diluent was divided into two groups, there were P0 (80% Tris
Aminomethane + 20% Egg Yolk); and P1 (80% Tris Aminomethane (without raffinose)
+ 20% Egg Yolk). Parameter of this research was motility percentage, viability
percentage, and abnormality percentage. Data of this research was analyzed
using paired design t test. The result showed that after five days chilled
preservation, was different (P<0,05) on the average motility percentage between
P0 (39,50±9,26%) and P1 (43,50±6,26%). There was significantly different
(P<0,01) on the average viability percentage between P0 (45,86±15,66%) and
P1 (46,38±15,32%). There was significantly different (P<0,01) on the average
abnormality percentage between P0 (1,76±0,60%) and P1 (2,31±0,75%). Total
motile sperm count after 5 days chilled preservation was different (P<0,05)
in P0 treatment and not different (P>0,05) in P1 treatment compared to the
expectation value of 40 million sperm/ml. The conclution of this research was
the tris aminomethane egg yolk diluent without raffinose could protect of Boer
goat semen quality during chilled preservation.
Keywords: boer goat semen,
tris aminomethane, raffinose, chilled preservation
Penulis: Abdul Rochim,
Muhammad Ade Salim, Nurul Isnaini, Trinil Susilawati
Kode Jurnal: jppeternakandd170078
