PENGARUH PENGGANTIAN BOVINE SERUM ALBUMIN (BSA) DENGAN PUTIH TELUR DALAM PENGENCER DASAR Cep-2 TERHADAP KUALITAS SEMEN KAMBING BOER PADA SIMPAN DINGIN
Abstract: The purpose of this
research wasinvestigated the substitution effect of Bovine Serum Albumin (BSA)
with albumen on CEP-2 diluent to semen quality of Boer goat that was stored at
3-50C. Research was conducted at Reproduction Laboratory and Field Laboratory
SumberSekarDau Animal Husbandry Faculty of Brawijaya University from December
2016until January 2017. The materials used for this research were fresh semen
from 3,5-4,5 years old Boer goat which was collected with artificial vagina.
Semen diluent was divided into two groups, those were P0 (90% CEP-2 + 10% Egg
Yolk) and P1 (90% CEP-2 (without BSA) + 0,4% albumen + 10% Egg Yolk). Data of
the research were analyzed using paired design t test. The result showed that
after eight days of chilled preservation, the sperm motility of P0 was not
significantly different with P1 (P>0,05). The average percentage of motility
during eight days preservation P0 was higher than P1. The viability between P0
and P1 showed highly significantly difference (P<0,01). The average
percentage of viability during eight days preservation P0 was higher than P1.
The abnormality between P0 and P1 showed highly significant difference
(P<0,01). The average percentage of abnormality between eight
dayspreservation P1 was higher than P0. Total motile sperms count after seven
days chilled preservation was not significantly different with hope value 40
million motile sperm/ml. The conclusion of this research was the substitution
of BSA with albumen could maintain Boer goat semen quality.
Keywords: CEP-2, Bovine
SerumAlbumin, Semen, Cryoprotectant
Penulis: Ayu Sulvi Istanty, M
Ade Salim, Nurul Isnaini, Trinil Susilawati
Kode Jurnal: jppeternakandd170071
