PENGARUH PENAMBAHAN DOSIS RAFINOSA DALAM PENGENCER SITRAT KUNING TELUR TERHADAP MOTILITAS, PERSENTASE HIDUP DAN ABNORMALITAS SPERMATOZOA SAPI ONGOLE
ABSTRACT: The research which
was conducted in the Regional Technical Services Unit Lampung Regional
Artificial Insemination Centres, Regency Terbanggi Besar, Central Lampung Regency, Lampung
Province on 19-20 May 2016, aims to determine the effect of dose rafinosa in
egg yolk citrate diluents on motility, percentage of live sperm and abnormal
spermatozoa Ongole. The experimental used
is completely randomized design with 6 treatments rafinosa dose (0.5%;
1%; 1.5%; 2%; 2.5%; 3%) in the egg yolk citrate diluent and each treatment be
repeated 4 times. The data obtained were analyzed using analyzed variety at
significance level of 5% or 1% then for variables that significantly undergone
orthogonal polynomial test to determine the optimum dose rafinosa. The results
showed that the addition of a diluent doses rafinosa in egg yolk citrate is not
significantly different (P> 0.05) on the percentage of live sperm in
prefreezing and post thawing motillity; on motility and sperm abnormalities in
the equilibration, prefreezing and PTM. Extra doses of raffinose in egg yolk
citrate highly significant (P <0.01) on the percentage living on
equilibration sermatozoa patterned quadratic. Found that the addition rafinosa
at equilibration was highly significant (P <0,01) to live spermatozoa with Ý
= 66.49 + 17,94x-4,23x2 with the optimum value of 2.1%.
Keywords: Rafinose , Motility, Percentage of Live,
Abnormal Spermatozoa, Ongole Cattle
Penulis: Sintha Pubiandara,
Sri Suharyati, Madi Hartono
Kode Jurnal: jppeternakandd160203