PENGARUH PENAMBAHAN DOSIS RAFINOSA DALAM PENGENCER TRIS KUNING TELUR TERHADAP MOTILITAS, PERSENTASE HIDUP DAN ABNORMALITAS SPERMATOZOA SAPI ONGOLE
ABSTRACT: The research was
conducted on May 16—18 at the Laboratory of Technical Services Unit Regional
Artificial Insemination Central Lampung, using semen from Ongole cattle. aims of this research were
determine the effect of different doses of rafinose and determine the optimal
doses rafinosa in tris yolk diluent for
motility, percentage of live and abnormalities spermatozoa Ongole cattle. The
experimental design used completely
randomized design with 6 treatments rafinosa doses (0,5%; 1,0%,
1,5%; 2,0%; 2,5%;
3%) in tris yolk diluent with 4 times replications. The data were
analyzed using analysis of varian with
5% or 1% significance level. Significantly different results was further tested
by orthogonal polynomials. The results
showed that the addition of rafinosa doses effect were not significantly
different (p>0.05) on percentage motility after prefreezing and Post Thawing
Motility, the percentage of live sperm Post Thawing Motility, and the
percentage of abnormal spermatozoa after equilibration, prefreezing, and Post
Thawing Motility, but the effect is very significantly (p<0.01) on
percentage motility spermatozoa after equlibration and percentage of live
spermatozoa after prefreezing. Addition doses of rafinose significant effect (p
<0.05) on the percentage of live sperm after equilibration. Addition doses
rafinose based pattem ortogonal polinomial regression on percentege motility
after equilibration with equations Ó¯ = 65,58-2,36x, the persentage of live
sperm after equilibration with aquations Ó¯=81,23-4,47x, and the persentage of live
sperm after prefreezing with aquations Ó¯=79,13-4,68x.
Keywords: Rafinosa , Egg Yolk
Tris, Motility, Percentage of Live, Abnormalities, Ongole Cattle
Penulis: Iis Nurlia, Sri
Suharyati, Madi Hartono
Kode Jurnal: jppeternakandd160197
