Pathogenic Assay of Probiotic Bacteria Producing Proteolytic Enzymes as Bioremediation Bacteria Against Vannamei Shrimp Larvae (Litopenaeus vannamei)
Abstract: Application of
bacteria in bioremediation of shrimp culture ponds is one of the methods used
to clean internal pollutants. This study aimed to evaluate the pathogenicity of
extracellular proteolytic enzyme produced by the probiotic bacteria as
bioremediation bacteria on vannamei shrimp larvae culture. There were five
probiotic bacteria, which were successfully isolated from the sediments served
as substrate in mangrove area. The isolated bacteria were coded in number as
13, 19, 30, 33, and 36. Pathogenic bacteria Vibrio harveyi was used as positive
control. Pathogenic assay was carried out in two different bacterial
concentrations, i.e. 10⁸ and 10⁶ cells.mL-1. The results showed that the lowest
survival rate (SR) of shrimp larvae in positive control V. harveyi was 53 and
65%. Whereas isolates with the highest SR value (100%) were obtained from
bacteria coded as 13 and 30. Isolates no. 19, 33 and 36 had SR of more than
90%. Total plate count (TPC) data showed that the bacteria increased
significantly at the end of the study with an average increase value of 24%.
The smallest TPC value was shown by bacterial isolate no. 19, while the largest
was obtained from the isolate no. 13. These results suggest that all probiotic
bacteria were not pathogenic to the vannamei shrimp larvae.
Keywords: aquaculture, shrimp,
bioremediation, pathogenesis, vibrio
Author: Wilis Ari Setyati,
Muhammad Zainuddin, Person Pesona Renta
Journal Code: jpperikanangg170042