Optimum Age of Starter for Microbial Transglutaminase (MTGase) Production Produced by Streptomyces thioluteus TTA 02 SDS 14 and Characterization of Crude Enzyme
Abstract: The condition of
starter plays important role in enzyme production. Optimum starter will lead to
the optimum yield of enzyme production. The experiments which were aimed to
obtain the optimum age for starter of
Streptomyces thioluteus TTA 02 SDS 14
from solid as well as liquid medium had been carried out which would be
used for transglutaminase production. Medium used for culture maintenance,
starter optimization and enzyme production was described by Bahrim. The result
showed that S. thioluteus TTA 02 SDS 14 was ready to be used as starter after
being cultured on solid medium continued
in liquid medium each for 6 days. The
enzyme production in a bioreactor using optimized starter produced enzyme with
the highest activity after being fermented for 2 days with 150 rpm agitation.
The crude enzyme active optimally at 45-50 oC, pH of 6 and no effect of metal ion and inhibitor on
enzyme activity.
Keywords: transglutaminase,
starter age, Streptomyces thioluteus
Author: Dewi Seswita Zilda,
Yusro Nuri Fawzya, Lia Siti Nur'amaliyah, Hana Nurullita Prestisia, Nisa
Rachmania Mubarik, Puspita Lisdiyanti
Journal Code: jpperikanangg170005
