Morphometrics and genetic diversity of Tegal, Magelang and their crossbred ducks based on Cytochrome b gene
Abstract: This experiment was
conducted to study the morphometric and genetic diversity of Tegal, Magelang,
and their crossbred ducks. Each ten female about twenty weeks old ducks from
Tegal, Magelang, Gallang, and Maggal ducks, respectively, were used as the
group materialss. The body weight, chest circumference, body length, shank
length, neck length, and pubis width were recorded and tested by analysis of
variance of one way classification as the morphometric characteristics.
Polymorphism of cytochrome b (cyt b) gene on mitochondrial DNA (mtDNA) was analyzed
by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
(PCR-RFLP). Genetic distance was analyzed based on value of heterozygosity,
whereas the phylogeny tree was reconstructed using MEGA6 software. The results
showed there were highly significant difference (P<0.01) on body weight,
chest circumference, body length, and neck length between population, while
shank and pubis width were not significant different. The genetic distance
between Gallang and Maggal ducks (0.206) was higher than Tegal and Magelang
ducks (0.169). It is concluded that the reciprocal crosses increased the
morphometric and genetic diversity of Indonesian local duck population.
Keywords: orphometric
diversity; genetic diversity; reciprocal crosses; local duck; cytochrome b
Author: H. Henrik, D.
Purwantini, I. Ismoyowati
Journal Code: jppeternakangg180002
