Methylenetetrahydrofolate Reductase C677T Distribution among Cervical Cancer Patients at Dr. Hasan Sadikin General Hospital
Abstract: Cervical cancer is
the second most common cancer among women in the world. Persistent infection
with high risk human papillomavirus (HPV) is one of the necessary causes of
cervical cancer development. However, host genetic factors may also play a role
in cervical cancer carcinogenesis. Methylenetetrahydrofolate reductase enzyme,
encoded by the MTHFR gene, regulates folate metabolism which is important for
genetic expression and stability. Single nucleotide polymorphism (SNP) C677T in
MTHFR gene may produce a thermo-labile enzyme, resulting in a reduced enzyme
activity. The aim of this study was to explore the SNP C677T of MTHFR gene and
the susceptibility to cervical cancer among cancer patients visiting Dr. Hasan
Sadikin General Hospital, Bandung, Indonesia. This descriptive quantitative
study involved cervical cancer patients recruited in 2010 and their control
group. Genomic DNA was extracted from patients’ blood. MTHFR C677T genotype was
performed using BeadXpress Reader Illumina® and some samples were re-genotyped
for confirmation using conventional PCR-RFLP. The distribution of MTHFR C677T
genotype in cervical cancer patients was 71.6%, 25.4%, and 3%, and 44%, 36%,
and 20% in control group for CC, CT, and TT, respectively. This yielded a
statistical significant difference of CC vs CT+TT (p 0.014 with OR 3.22 and CI
95% 1.24 – 8.33). Taken together, this result indicates that T allele has a
protective effect against cervical cancer development. Further studies to confirm
this effect in bigger population is warranted. [MKB. 2016;48(4):216–21]
Key words: Cervical cancer,
MTHFR C677T, polymorphism, Bandung
Penulis: Ani Melani Maskoen,
Cynthia Kurniawan, Herman Susanto, Edhyana Sahiratmadja
Kode Jurnal: jpkedokterandd160317
