KUALITAS SEMEN CAIR SAPI SIMENTAL MENGGUNAKAN LARUTAN ISOTONIS KOMERSIAL PADA KONSENTRASI DAN LAMA PENYIMPANAN BERBEDA
Abstract: Artificial
insemination (AI) is a technique to insert the sperm or semen of bull thawed
and processed first into the genital tract of female methods and a special tool
called insemination gun, efforts to optimize the management of the cement in
order to obtain the quality of cement is optimal to do with the selection of
diluent cement. One alternative materials cement diluent is an isotonic
solution Commercial (LIK). This study aimed to examine the interaction between
the concentration and duration of storage of diluent solution isotonic
different to the quality of spermatozoa cow simental. This research was
conducted at the Regional Technical Implementation Unit of Artificial
Insemination Centres (UPTD BIB) Tuah Sakato, Payakumbuh. In April 2016. This
study used a randomized block design (RAK) factorial design to Factor A
(Concentration LIK) ie A1 = 100 ml Tris Egg Yolk, A2 = (60 ml) Tris Egg Yolk +
(40 ml) LIK, A3 = (55 ml) Tris Egg Yolk + (45 ml) LIK, A4 = (50 ml) Tris Egg
Yolk + (50 ml) LIK, and factor B (retention) is B1 = 0 day, B2 = 1, B3 = 2 day,
days, B4 = 3 days. Each treatment consists of three replicates. Parameters
measured were motility, percentage of survival, abrnormalitas, and MPU. These
results indicate that an isotonic solution can be used as an alternative
Commercial diluent with the best results on the percentage LIK 40 ml, but the
effectiveness of the efficiency of storage use was only on the first day (24
hours).
Keywords: Catle simental;
Commercial isotonic solution; motility; percentage of survival; abnormalities
and Plasma Membrane Whole (MPU)
Penulis: Bayu Anke Mardian,
Zumarni, Anwar Effendi Harahap
Kode Jurnal: jppeternakandd170132
