Knock-out transmembrane prostate androgen-induced protein gene suppressed triple-negative breast cancer cell proliferation
Abstract: Triple negative
breast cancer (TNBC) tends to grow more rapidly and has poorer prognosis
compared to others. High expression of transmembrane prostate androgen-induced
protein (TMEPAI) correlates with poor prognosis in TNBC patients. However, the
mechanistic role of TMEPAI in tumorigenic remains unknown. This study aimed to
knock-out TMEPAI in TNBC cell line to determine its function further in cells
proliferation.
Methods: CRISPR-Cas9 has been used previously to knock-out TMEPAI in
Hs857T TNBC cell line. Hs587T TNBC parental cell line (wild-type/WT) and TMEPAI
knock out Hs 586T cell lines were cultured in Dulbecco’s modified eagle medium
(DMEM) supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin and
amphotericin B. Both cell lines were seeded in 24-well plates and counted every
two days, then proliferation rates were plotted. Afterwards, total RNA were
isolated from the cells and Ki-67, and TGF-β mRNA expression levels as
proliferation markers were determined.
Results: Cell proliferation rates as displayed in growth curve plots
showed that WT-TMEPAI cell line grew more rapidly than KO-TMEPAI. In
accordance, mRNA expression levels of
Ki-67 and TGF-β were
significantly decreased KO-TMEPAI as compare to TMEPAI-WT.
Conclusion: Knock-out of TMEPAI attenuates cell proliferation in TNBC.
Keywords: proliferation;
TGF-β; TMEPAI; triple negative breast cancer
Author: Bantari W.K. Wardhani,
Meidi U. Puteri, Yukihide Watanabe, Melva Louisa, Rianto Setiabudy, Mitsuyasu
Kato
Journal Code: jpkedokterangg170225
