Isolation of Lactic Acid Bacteria with Cholesterol-Lowering Activity from Digestive Tracts of Indonesian Native Chickens
Abstract: The aim of the study
was to identify the cholesterol-lowering activity of indigenous lactic acid
bacteria isolated from the small intestine, cecum, and colon of Indonesian
native chickens and evaluated for bile salt hydrolase (BSH) activity in vitro
by using MRS media added taurodeoxycholic acid (TDCA) and CaCl2. The
quantitative measurement of cholesterol-lowering activity of LAB was
investigated by using soluble cholesterol containing MRS broth (100 µg/mL of
cholesterin) and incubated at 37 °C for 48 h. Cholesterol content in
supernatant was analyzed using microplate reader. The highest percentage of
cholesterol reduction found in isolates from colon of native chicken with the
value of 17.43% and identified as Lactobacillus plantarum. Based on
phylogenetic tree analysis, this isolate was closely related to L. plantarum
strain LGFCP4 (accession number KM199683.1) isolated from GIT of Guinea fowl
from India. It could be concluded that L. plantarum AKK-30 had
cholesterol-lowering activity.
Keywords: lactic acid bacteria
(LAB); cholesterol; bile salt hydrolase (BSH); native chicken
Author: H. Julendra, Ade Erma
Suryani, Lusty Istiqomah, Ema Damayanti, Muslih Anwar, Naditya Fitriani
Journal Code: jppeternakangg170030
