ISOLASI, SELEKSI DAN OPTIMASI BAKTERI PENGHASIL FITASE DARI TANAH VULKANIK DI GUCI, TEGAL
Abstrak: The purpose of this
study was to isolated, selected and characterized phytase bacteria derived from
volcanic soil in the Guci, Tegal. Bacteria from the volcanic soil was isolated
and selected on Luria Betany (LB) media containing 4% phytic acid. Selected
colonies were optimized by physical character with determining the relative
activity. Colonies of bacteria could be isolated from the volcanic soil,
selected, and had the highest relative activity in colonies of TG3 and TG3-5.
TG3-5 bacterial colonies producing phytate had the highest optimum value at a
temperature of 55°C, pH 7, the incubation time of 90 minutes, the substrate
concentration of 5% and a best metal cofactor on Zn2+ (10-4M). Volcanic soil in
the Guci, Tegal containing phytase-producing bacteria with certain physical
characteristics.A
Kata Kunci: Phytase-producing
bacteria, volcanic soil, physical characteristics, the relative activity
Penulis: Adi Magna Patriadi
Nuhriawangsa, Adi Ratriyanto, Sajidan, Catur Suci Purwati
Kode Jurnal: jppeternakandd160415