Identifikasi Keragaman Gen Pituitary Transcription Factor 1 (Pou1f1) Pada Kambing Peranakan Etawah (Pe) di BPTU KDI-HPT Pelaihari
Abstract: Pituitary
Tanscription Factor 1 (POU1F1) has been identified as the pituitary spesific
transcription factor that regulates the expression of the growth hormone (GH)
and prolactin (PRL) in the anterior pituitary. The objective of this research
is identification the polymorphism of POU1F1 gene of etawah grade goat in BPTU
KDI-HPT Pelaihari South Kalimantan use Polymerase Chain Reaction- Restriction Fragment
Length Polymorphism (PCR-RFLP). PCR products of POU1F1 digested with Hinf1
enzyme which restric G|AnTC. Samples are
used as the material of DNA from blood samples of 115 PE goats. POU1F1 gene amplification was performed with
annealing temperature of 62 oC for 20 seconds resulting in a fragment with the
length of 601 bp which is located at intron 5 and exon 6. The result of
PCR-RFLP analysis indicated that the POU1F1 gene was monomorphic. B allele had 3 fragments at 275, 243, and 83
bp respectively.
Keywords: Etawah grade goat;
PCR-RFLP; Pituitary Tanscription Factor 1 (POU1F1)
Penulis: A. B. P Suparman, R. H
Mulyono, Jakaria
Kode Jurnal: jppeternakandd150409