Identification of Carotenoids in Halimeda macroloba Reef Associated Bacteria
Abstract: Several carotenoid
pigments which are produced by sea microorganisms are shown to function as
antimicrobe compounds and potential antioxidants. Coral reefs with high levels
of diverse biota facilitate Halimeda as a primary component and producer to
have associated bacteria that produce relatively unknown metabolic compounds.
This research attempts to isolate bacteria that are associated with Halimeda
macroloba, identify it, as well as analyze the pigment content produced. A
yellow-orange bacteria colony is successfully isolated and given the temporary
name MK_HM. This bacteria is in the shape of circular (oval) rods and are
gram-variable bacteria. Based on the sequencing analytical results, Blast
homology, as well as a phylogenetic analysis, it shows that the bacteria have a
relationship with Exiguobacterium aestuarii TF-16 at 94%, so that it can be strongly
predicted that the MK_HM strain is a new species. This pigment analysis, which
is conducted on the Exiguobacterium sp. MK_HM bacteria extract, reveals that
this strain produces carotenoid pigments of diadinochrome, semi-α-carotenone,
dinoxanthin, and P457.
Keywords: Exiguobacterium,
carotenoid, diadinochrome, semi-α-carotenone, dinoxanthin, and P457
Author: Wiwik Astuti, Ocky
Karna Radjasa, Ferry Fredy Karwur, Ferdy S. Rondonuwu
Journal Code: jpperikanangg160070
