Identification of BMP15 Exon 2 for fecundity traits by PCR-RFLP and nucleotide sequencies in Kejobong goat
Abstract: Kejobong goat is
known as prolific and high productivity goat breed in Indonesia. PCR-RFLP and
sequencing technique was established in the present study to accomplish the
polymorphisms of Bone Morphogenetic Protein 15 (BMP15) gene exon 2 on Kejobong
goat does. The blood samples was collected from 48 Kejobong does which were
selected based on their litter size. The size of PCR amplification of BMP15
gene exon 2 was 837 bp. The product of PCR-RFLP technique digested by HinfI
enzyme showed that the samples were monomorphic. Authentication result using
nucleotide sequencing found 4 substitution (A391G, C464G, T828C and C830G), 1
alignment gap (site 817) and 1 insertion nucleotide (site 822). This mutations
caused 6 haplotypes formatted. The mutants of BMP15 exon 2 on Kejobong goats
indicated that this breed had their own mutation controling the prolific trait.
The phylogenetic tree build on the sequences of BMP15 gene exon 2 of Kejobong
goats was grouped into 3 clusters. The alignment gap indicated to be the specific
marker for the prolific trait (duplet) in Kejobong goat. The particular
insertion site could be the recognition site of Kejobong goat based on BMP15
exon 2.
Keywords: fecundity trait;
polymorphisms; BMP15 gene; prolific; Kejobong goat
Author: A. Febriana, S.
Sutopo, E. Kurnianto
Journal Code: jppeternakangg170045
