Hepatoprotective Effect of Trigona spp. Bee Propolis against Carbon Tetrachloride-Induced Liver Injury in Rat
Abstract: Oxidative stress
reaction can cause liver injury. This process can be prevented by antioxidant
activities which can break the destructive chain caused by free radical
substances in the liver. Propolis produced by Trigona spp. bee is known to have
a high level of antioxidant. The aim of this study was to examine the effect of
Trigona spp. bee propolis on liver histological toxicity caused by carbon
tetrachloride-induced oxidative stress.
Methods:This experimental study was conducted in September 2013 at the
Animal Laboratory of Departement of Pharmacology and Therapy, Faculty of
Medicine Universitas Padjadjaran. Twenty-four healthy male Wistar rats as
objects were adapted for one week and randomly divided into 3 groups. Group I
was the control negative, group II was given carbon tetrachloride on day 14,
group III was given Trigona spp. bee propolis on day 1-14. On day 14, group III
was injected CCl4 intraperitoneally. The quantitative data were statistically
analyzed using the one way ANOVA and Tukey test with p value < 0.05.
Results: Group I showed the liver contained normal cells, without
significant injury of the membrane, round and complete nucleus. The average
number of liver cell was 464 ± 9.59281 cells/field; group II underwent necrosis
and the average of the cells was 146 ± 7.56885 cells/field; group III showed
some normal liver cells, and some necrotic area with the normal liver cells
average was 263 ± 14.10860 cells/field. The p-value=0.00.
Conclusions: Trigona spp. bee propolis has a hepatoprotective effect
against CCl4-induced liver injury histologically.
Keywords: Carbontetrachloride,
hepatoprotective, propolis, Trigona spp.
Author: Rachel Amelia,
Achadiyani Achadiyani, M. Begawan Bestari
Journal Code: jpkedokterangg160255
